Determination of Protein Molecular Weight and Size Using the Agilent 1260 Infi nity Multi-Detector Bio-SEC Solution with Advanced Light Scattering Detection
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چکیده
This Application Note shows the determination of protein molecular weight and size, together with linear quantifi cation using the Agilent 1260 Infi nity Multi-Detector Bio-SEC Solution. The molecular weights and sizes of proteins, ranging from cytochrome C (11 kDa) to thyroglobulin (670 kDa) were correctly determined, independent of sample loading. Using the dual angle light scattering detector enables high sensitivity aggregation determination when compared to UV detection. Determination of Protein Molecular Weight and Size Using the Agilent 1260 Infi nity Multi-Detector Bio-SEC Solution with Advanced Light Scattering Detection
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تاریخ انتشار 2014